雙通道實時定量PCR 儀
【介紹】
DNA Engine Option 2 是一款雙波長實時PCR 儀。96 個藍-綠 LED 順序激發(fā)樣品,通過二個光電二極管來檢測。兩個激發(fā)波長范圍: 一為523–543nm(適用于FAM、SYBR Green I 等),還有一個是 540–700nm(適用于普遍使用的HEX、TEMRA、TET、VIC 等熒光素)。
DNA Engine Option 2 既可以支持普通的單重PCR,也可以支持多重 PCR。多重 PCR 除了可以節(jié)省試劑和時間外,還可以使內對照的使用成為可能,從而對樣品PCR 效率有更進一步的掌控。此外,多重PCR 也 使基因表達分析更為簡便。其具體性能如下:
一次96 孔熒光檢測時間在3 秒之內,最大程度減少了熒光交叉
完美的溫度控制性能和梯度PCR 功能
Opticon Monitor 分析軟件可以非常簡單地設置RT–PCR、多重PCR 等反應,以及DNA 定量、基因表達、等位基因等功能分析
LED 陣列與反應板一一對應,保證激發(fā)光準確進入目標管。發(fā)射光通過光電倍增管(PMTs)系統(tǒng)進行信號放大。
【技術指標】
Base thermal cycler |
DNA Engine |
Sample capacity |
96-well PCR plate (low-profile) or 12 x 0.2 ml 8-tube strips (low-profile) |
Sample volume |
10–100 µl (20 µl recommended) |
Dimensions (W x D x H) |
34 x 47 x |
Weight |
|
Fluorescence excitation range |
470–505 nm |
Fluorescence detection range |
Channel 1: 523–543 nm |
Linear dynamic range of starting copy number |
Up to 10 orders of magnitude |
Detection limit of template starting copy number |
Down to single initial template copy detection |
Gradient Specifications | |
Gradient range |
30– |
Temperature differential range |
1– |
Gradient accuracy |
± |
Temperature uniformity |
± |
Gradient calculator accuracy |
± |
Computer System Requirements (Computer NOT Included With System) | |
Model |
Desktop tower computer |
PCI card slot dimensions |
Full-size card, |
Operating system |
Windows XP Professional |
Storage capacity |
40 GB hard drive |
Memory |
512 MB RAM |
Port |
Communication (COM) |
Supplied With System | |
Data acquisition board |
National Instruments PCI-6036E, 200 x 10³ samples/sec |
Note: Specifications subject to change without notice. |